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集菌儀實驗與操作步驟

來源: 發布時間:2018-04-09

集菌(jun)儀的操作過(guo)程(cheng):把儀器的活動泵柄和鉤松開,將培(pei)育器的軟管正確(que)放入活動泵的定位槽內,鎖緊活動泵柄及鉤。在中止狀況時,按一(yi)下“RUN/STOP”鍵或踩動一下腳踏(ta)開(kai)關,儀器即發動,顯現(xian)屏上顯現(xian)儀器輸出(chu)轉速;而(er)儀器轉運的快慢,可根據(ju)需要在儀器作業時,經過“UP”、“DOWN”鍵“30R”、“60R”、“100R”“160R”“220R”檔(dang)位鍵隨時調整,最(zui)高(gao)轉速為220rpm,最(zui)低有用轉速為30rpm。在粉針劑溶解(jie)時,主張運(yun)用(yong)轉速在30rpm上下(可直接(jie)按“30R”鍵發動);在大容量打針劑過濾操(cao)作(zuo)時(shi),主張運用(yong)轉速(su)為160rpm上(shang)下(可直接按“160R”鍵發動);然后根據詳細需要做微(wei)調,運用“UP”、“DOWN”鍵(jian)作上下調整。當替換檢品和完結過濾時,再按一下“RUN/STOP”鍵或踩動(dong)一下腳踏開關(guan),儀(yi)器即中止(zhi)工作(zuo)。

 

試品經(jing)(jing)過進樣管道接(jie)連被(bei)注入集菌培育器(qi)中(zhong),運用(yong)集菌培育器(qi)內構成(cheng)的下壓(ya),經(jing)(jing)過0.45微(wei)米孔徑的(de)濾膜(mo)過(guo)濾,供(gong)試品中可能(neng)存(cun)在的(de)微(wei)生(sheng)物(wu)(wu)被(bei)截留搜集(ji)在濾膜(mo)上,集(ji)菌(jun)儀經過(guo)沖刷濾膜(mo)除掉供(gong)試品的(de)抑菌(jun)成(cheng)分。然后把所需(xu)培(pei)育基經過(guo)進(jin)(jin)樣管道直接注入集(ji)菌(jun)培(pei)育器中,放置規則的(de)溫度(du)培(pei)育,調查(cha)是(shi)否有長(chang)菌(jun)現象。技(ji)能(neng)特色:不銹鋼機箱滿(man)意實(shi)驗者對無菌(jun)實(shi)驗室的(de)要求;相位壓控(kong)(kong)無級(ji)調速操作操控(kong)(kong)便利;渦輪(lun)減速系統,下降機器作業噪音,增(zeng)大扭距力保證工(gong)作安全(quan);渦輪(lun)減速系統動力系統選用(yong)(yong)(yong)高(gao)功率動力,保證高(gao)粘度(du)樣品實(shi)驗的(de)進(jin)(jin)行(xing)并進(jin)(jin)步速度(du)。產(chan)品用(yong)(yong)(yong)途:廣泛適用(yong)(yong)(yong)于(yu)打針用(yong)(yong)(yong)無菌(jun)制(zhi)劑的(de)無菌(jun)檢測,包含抗(kang)生(sheng)素類及含有抑菌(jun)成(cheng)份(fen)的(de)藥品、大輸液(ye)、水針劑、滅菌(jun)醫療(liao)用(yong)(yong)(yong)具、無菌(jun)打針用(yong)(yong)(yong)水等,也可用(yong)(yong)(yong)于(yu)食物(wu)(wu)、飲(yin)料職(zhi)業等微(wei)生(sheng)物(wu)(wu)極限查(cha)看。

 

集菌(jun)儀(yi)(yi)注意(yi)事(shi)項:儀(yi)(yi)器(qi)(qi)(qi)有(you)必要(yao)有(you)用(yong)接地(di);若無菌(jun)室選用(yong)化學消毒劑消毒時(shi),應(ying)(ying)(ying)將(jiang)(jiang)儀(yi)(yi)器(qi)(qi)(qi)置于密封罩內,或(huo)搬(ban)出無菌(jun)室,防止損壞電子部(bu)件(jian)和腐蝕金(jin)屬配件(jian);應(ying)(ying)(ying)堅持取(qu)樣(yang)(yang)針上的過(guo)(guo)濾膜枯燥(zao),氣流(liu)疏(shu)通,正常(chang)進(jin)(jin)(jin)液(ye)。辦法(fa)是在取(qu)樣(yang)(yang)針刺進(jin)(jin)(jin)瓶(ping)(ping)裝流(liu)體樣(yang)(yang)品(pin)時(shi),應(ying)(ying)(ying)先開(kai)機(ji),然(ran)后倒轉容器(qi)(qi)(qi)瓶(ping)(ping);替換檢品(pin)或(huo)完結過(guo)(guo)濾時(shi),應(ying)(ying)(ying)停機(ji),不然(ran)會使(shi)培育器(qi)(qi)(qi)內發生(sheng)過(guo)(guo)高(gao)的氣壓,使(shi)膠帽彈出,影響運用(yong)。可摘下(xia)膠帽放(fang)氣,消除(chu)培育器(qi)(qi)(qi)的壓力;若進(jin)(jin)(jin)液(ye)管內呈(cheng)現過(guo)(guo)多氣泡,應(ying)(ying)(ying)將(jiang)(jiang)活動(dong)(dong)泵轉速下(xia)降,并查看(kan)進(jin)(jin)(jin)氣濾膜是否被浸濕(shi),若不能進(jin)(jin)(jin)液(ye),則查看(kan)進(jin)(jin)(jin)液(ye)針管是否疏(shu)通;儀(yi)(yi)器(qi)(qi)(qi)工作時(shi)切勿將(jiang)(jiang)手(shou)伸入活動(dong)(dong)泵內,待扣上活動(dong)(dong)泵柄和鉤后再開(kai)機(ji);替換或(huo)緊(jin)固轉輪時(shi)有(you)必要(yao)封閉(bi)儀(yi)(yi)器(qi)(qi)(qi)電源(yuan)操作;防止將(jiang)(jiang)轉輪進(jin)(jin)(jin)行(xing)高(gao)溫(wen)或(huo)濕(shi)熱滅菌(jun),防止轉輪滋潤消毒,液(ye)體進(jin)(jin)(jin)入轉輪時(shi)應(ying)(ying)(ying)及時(shi)擦(ca)洗潔凈;本機(ji)具有(you)活動(dong)(dong)泵安全保(bao)護功用(yong),當翻開(kai)活動(dong)(dong)泵動(dong)(dong)塊時(shi),儀(yi)(yi)器(qi)(qi)(qi)會主動(dong)(dong)中止工作,合(he)上后需按(an)RUN/STOP”鍵或踩動(dong)一下腳(jiao)踏(ta)(ta)(ta)開關(guan)(guan)康復工作;防(fang)止(zhi)接連踩動(dong)腳(jiao)踏(ta)(ta)(ta)開關(guan)(guan);踩動(dong)腳(jiao)踏(ta)(ta)(ta)開關(guan)(guan)后,腳(jiao)不要當(dang)即(ji)松開,應適當(dang)推(tui)遲(chi)至儀器發動(dong)或中(zhong)止(zhi);防(fang)止(zhi)將腳(jiao)踏(ta)(ta)(ta)開關(guan)(guan)置于具有(you)強腐(fu)蝕性(xing)、氧(yang)化性(xing)的環境;防(fang)止(zhi)將腳(jiao)踏(ta)(ta)(ta)開關(guan)(guan)內部進入(ru)液體;防(fang)止(zhi)液體進入(ru)機殼(ke)。

 

集菌(jun)儀(yi)保護(hu):儀(yi)器(qi)作業環(huan)境要符合要求(qiu),以(yi)保證儀(yi)器(qi)的(de)運用(yong)(yong)(yong)(yong)壽命,請定(ding)(ding)時(shi)(shi)對(dui)其進(jin)(jin)行保護(hu)和查看;儀(yi)器(qi)運用(yong)(yong)(yong)(yong)結束后,對(dui)外(wai)表進(jin)(jin)行擦洗(xi),堅持清(qing)潔;定(ding)(ding)時(shi)(shi)對(dui)活動(dong)泵、排液槽進(jin)(jin)行清(qing)洗(xi),可脫卸部位(除轉輪外(wai))可進(jin)(jin)行濕熱滅(mie)(mie)菌(jun);定(ding)(ding)時(shi)(shi)對(dui)動(dong)塊軸處進(jin)(jin)行清(qing)潔,特別(bie)是有液體進(jin)(jin)入(ru)時(shi)(shi)。菌(jun)儀(yi)是集菌(jun)培育器(qi)的(de)配套運用(yong)(yong)(yong)(yong)儀(yi)器(qi),經過(guo)集菌(jun)儀(yi)的(de)定(ding)(ding)向活動(dong)加壓(ya)效果,供試(shi)品被正壓(ya)過(guo)濾并(bing)在濾器(qi)內進(jin)(jin)行培育,以(yi)查驗(yan)供試(shi)品是否含菌(jun)。可廣泛適用(yong)(yong)(yong)(yong)于打(da)針用(yong)(yong)(yong)(yong)無菌(jun)制劑的(de)無菌(jun)檢測,包(bao)含抗生(sheng)素類及(ji)含有抑菌(jun)成(cheng)分的(de)藥品、大輸液、水針劑、滅(mie)(mie)菌(jun)醫療用(yong)(yong)(yong)(yong)具、無菌(jun)打(da)針用(yong)(yong)(yong)(yong)水等,亦(yi)可用(yong)(yong)(yong)(yong)于食物、飲(yin)料職(zhi)業等微(wei)生(sheng)物極(ji)限查看。

 

樹(shu)立最謹慎的(de)食(shi)物(wu)(wu)安(an)(an)(an)全(quan)(quan)標(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)系統。加速制修(xiu)訂工(gong)業(ye)(ye)展(zhan)開和監(jian)管急需的(de)食(shi)物(wu)(wu)基礎標(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)、產品標(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)、配套查驗辦法(fa)標(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)、生(sheng)產經(jing)營衛生(sheng)標(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)等。加速制修(xiu)訂重金屬、農藥殘留(liu)、獸(shou)藥殘留(liu)等食(shi)物(wu)(wu)安(an)(an)(an)全(quan)(quan)標(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)。親近盯梢世界標(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)展(zhan)開更新狀況,整合現有(you)資源樹(shu)立掩蓋(gai)世界食(shi)物(wu)(wu)法(fa)典(dian)及(ji)有(you)關發達國(guo)(guo)家食(shi)物(wu)(wu)安(an)(an)(an)全(quan)(quan)標(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)、技(ji)能(neng)(neng)法(fa)規的(de)數據庫,展(zhan)開世界食(shi)物(wu)(wu)安(an)(an)(an)全(quan)(quan)標(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)比(bi)較研究。加強標(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)盯梢點評和宣揚遵循訓練。鼓舞(wu)食(shi)物(wu)(wu)生(sheng)產企業(ye)(ye)擬定嚴于(yu)(yu)食(shi)物(wu)(wu)安(an)(an)(an)全(quan)(quan)國(guo)(guo)家標(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)、當(dang)地標(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)的(de)企業(ye)(ye)標(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun),鼓舞(wu)職業(ye)(ye)協會擬定嚴于(yu)(yu)食(shi)物(wu)(wu)安(an)(an)(an)全(quan)(quan)國(guo)(guo)家標(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)的(de)集體標(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)。依托(tuo)現有(you)資源,樹(shu)立食(shi)物(wu)(wu)安(an)(an)(an)全(quan)(quan)標(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)網上公開和查詢渠道,發布一切食(shi)物(wu)(wu)安(an)(an)(an)全(quan)(quan)國(guo)(guo)家標(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)及(ji)其(qi)他相(xiang)關標(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)。整合建造監(jian)測(ce)抽檢數據庫和食(shi)物(wu)(wu)毒理(li)學數據庫,進步標(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)基礎研究水平。將構成技(ji)能(neng)(neng)標(biao)(biao)(biao)準(zhun)(zhun)(zhun)(zhun)(zhun)作(zuo)為安(an)(an)(an)排施(shi)行相(xiang)關科研項目(mu)的(de)重要(yao)目(mu)標(biao)(biao)(biao)之一,并(bing)列入食(shi)物(wu)(wu)科研重要(yao)查核目(mu)標(biao)(biao)(biao),相(xiang)關成果能(neng)(neng)夠作(zuo)為專業(ye)(ye)技(ji)能(neng)(neng)資格評定根據。

 

進(jin)步農業(ye)(ye)標(biao)(biao)準化(hua)(hua)(hua)水(shui)平。施行農業(ye)(ye)標(biao)(biao)準化(hua)(hua)(hua)推行工程,推行杰出農業(ye)(ye)標(biao)(biao)準。持續推進(jin)農業(ye)(ye)標(biao)(biao)準化(hua)(hua)(hua)示范(fan)區、園藝作物標(biao)(biao)準園、標(biao)(biao)準化(hua)(hua)(hua)規(gui)劃養殖場(小區)、水(shui)產健康養殖場建(jian)(jian)造(zao)。支(zhi)持(chi)杰出農(nong)業標準認證品(pin)(pin)(pin)牌農(nong)產品(pin)(pin)(pin)展(zhan)開(kai),進步安(an)全(quan)優質品(pin)(pin)(pin)牌農(nong)產品(pin)(pin)(pin)比重。樹立(li)健全(quan)畜禽屠(tu)宰管(guan)理制(zhi)(zhi)(zhi)度,加速(su)推進病死畜禽無害化(hua)處理與養殖業穩妥聯動機制(zhi)(zhi)(zhi)建(jian)(jian)造(zao),加強病死畜禽、屠(tu)宰廢棄物無害化(hua)處理和(he)資(zi)源化(hua)運用(yong)。加強糧食(shi)質量(liang)安(an)全(quan)監測與監管(guan),推進樹立(li)重金屬等超支(zhi)糧食(shi)處置長(chang)效機制(zhi)(zhi)(zhi)。推進農(nong)產品(pin)(pin)(pin)生產者積極(ji)參(can)與國家農(nong)產品(pin)(pin)(pin)質量(liang)安(an)全(quan)追溯(su)管(guan)理信(xin)息渠道工(gong)作(zuo)。展(zhan)開(kai)肉(rou)類、蔬(shu)菜等產品(pin)(pin)(pin)追溯(su)系統建(jian)(jian)造(zao)的地區要加速(su)樹立(li)高效工(gong)作(zuo)長(chang)效機制(zhi)(zhi)(zhi)。

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